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Showing: 1221 - 1240 of 3934 results

  4. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  6. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-434022

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  10. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Article
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Use
    Tags
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61628-plasmid-map-sequence-id-396550

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  12. T7-VEE-IRES-Puro

    Plasmid
    #58970
    Purpose
    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Type
    Empty backbone
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58970-plasmid-map-sequence-id-91400

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  14. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Article
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52255-plasmid-map-sequence-id-80847

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  15. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    His6-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11520-plasmid-map-sequence-id-324017

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  19. GFP::L4440

    Plasmid
    #11335
    Depositor
    Guy Caldwell
    Insert
    GFP
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11335-plasmid-map-sequence-id-237574

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  20. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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Showing: 1221 - 1240 of 3934 results