-
Plasmid#206935PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterMP-84Available sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MP-84-spCas9-U6-gRNA-tlock
Plasmid#206936PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterMP-84Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1pr_v1-RT
Plasmid#97087PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.DepositorInsertnuclear paraspeckle assembly transcript 1 (NEAT1 Human)
UseCRISPRTagsEYFPExpressionMutationPromoterCMVAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
BlatCas9
Plasmid#163792PurposeThe plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LT0
Plasmid#210551PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporterDepositorInsertpU6::AAVS1gRNA::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT1
Plasmid#210552PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporterDepositorInsertpU6::AAVS1gRNA::LT1::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT2
Plasmid#210553PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporterDepositorInsertpU6::AAVS1gRNA::LT2::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Stuffer
Plasmid#106248PurposeDestination vector for U6::gRNA expression cassetteDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc2
Plasmid#134451PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
px458_Conc2
Plasmid#134449PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc3
Plasmid#134458PurposeEmpty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
px458_Conc3
Plasmid#134450PurposeEmpty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pARS3_MUbCAS9_MC
Plasmid#128767PurposeT-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNADepositorTypeEmpty backboneUseTagsHAExpressionPlantMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC0_6_sgRNA
Plasmid#163717PurposePlasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)DepositorInsertsgRNA expression cassette
UseSynthetic Biology; Moclo vector level 0 (type 234)TagsExpressionMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9
Plasmid#135010PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9DepositorInserthumanized S. pyogenes Cas9
UseTags3xFLAG (N terminal on insert)ExpressionMammalianMutationPromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti dCAS-VP64_Blast
Plasmid#61425Purpose3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)DepositorHas ServiceLentiviral PrepInsertdCAS9(D10A, N863A)-VP64_2A_Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only