Addgene

We narrowed to 14,386 results for: invs

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pCDNA_MET_3Flag

Plasmid

#182496

Purpose

Contains cDNA of wt MET

Depositor

Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

MET (MET Human)

Use

Tags

C-terminal 3xFlag tag

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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TurboID-MCS MSCV-IRES-GFP

Plasmid

#207958

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDLX5/6

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM3-Flag

Plasmid

#180408

Purpose

lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM3 (TGM3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Express tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ihSyn1-tTA

Plasmid

#99120

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

ihSyn1

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

Plasmid

#199585

Purpose

Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Use

Tags

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Promoter

Available since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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DNM2 delta PRD

Plasmid

#168275

Purpose

Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

DNM2 (DNM2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa 1-742

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PPP1R12C wt

Plasmid

#31659

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)

Use

Tags

M2

Expression

Mammalian

Mutation

codon-optimized

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only