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Plasmid#60021PurposepARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only
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pCRUSH
Plasmid#54480PurposeMouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puroDepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and RNAiTagsSA-EGFP-polyA, U6 promoter, loxP, and pgk-puroExpressionMammalianMutationPromoterU6Available sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRUSH
Plasmid#54479PurposeMouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sitesDepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and RNAiTagsSA-EGFP-polyA, U6 promoter, loxP, and pgk-neoExpressionMammalianMutationPromoterU6Available sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorInsertCas9
UseTagsFlag, NLS, and polyA tailExpressionMammalianMutationPromoterCAGAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN479
Plasmid#86234PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locusDepositorInsertspCas9-nickase and sgRNA against mouse rosa26 locus
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE. The vec…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272
Plasmid#22732DepositorInsertLox71-MCS-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE containi…PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMCS.DT-A
Plasmid#22730DepositorInsertDT-A
UseBac recombineeringTagsExpressionMutationThis is a gap-repair vector designed for retrievi…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
LRG-2.1T-neo-sgROSA
Plasmid#105587Purposelentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistanceDepositorInsertgRNA targeting rosa26 locus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330_Rosa_sgRNA
Plasmid#97007PurposeExpresses Cas9 and Rosa26 locus specific sgRNADepositorInsertRosa26 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCA.66/2272
Plasmid#22733DepositorInsertLox66-pU(delta)TK-EM7Neo-Lox2272
UseCre/Lox; Bac recombineeringTagsExpressionMutationThis vector was designed for assembling gene targ…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSwith-tdTomato
Plasmid#60953PurposeCre recombinase-mediated cassette exchangeDepositorInserttdTomato
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai65(RCFL-tdT) targeting vector
Plasmid#61577PurposeTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertpCAG-FSF-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai66(RCRL-tdT) targeting vector
Plasmid#61578PurposeTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertCAG-RSR-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai95(RCL-GCaMP6f) targeting vector
Plasmid#61579PurposeTarget a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locusDepositorInsertCAG-LSL-GCaMP6-fast
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-sgR26-eCas9-T2A-BlastR
Plasmid#172493PurposeFor CRISPR-assisted HDR, Rosa26 locusDepositorInserteCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-tdTomato
Plasmid#180154PurposetdTomato dre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseMouse Targeting; Dre/roxTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
R26/P-nlsrtTA
Plasmid#46183DepositorInsertsnlsrtTA
pgk-puro
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only