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Showing: 101 - 120 of 461 results

  2. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid
    #162918
    Purpose
    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162918-plasmid-map-sequence-id-330526

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  15. pAS-1 (perf)

    Plasmid
    #40836
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–perf
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pAS-2 (mm21)

    Plasmid
    #40837
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  19. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  20. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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Showing: 101 - 120 of 461 results