Addgene

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pJMP2480

Plasmid

#160080

Purpose

Z. mobilis CRISPRi, promoter C, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-dCer3

Plasmid

#191328

Purpose

Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

dCer3-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mScarlet

Plasmid

#191324

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mScarlet (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS713

Plasmid

#188885

Purpose

Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2132

Plasmid

#160075

Purpose

Z. mobilis CRISPRi, promoter B, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP2046

Plasmid

#160073

Purpose

Z. mobilis CRISPRi, promoter A, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pNM220_AAVS1 integration helper

Plasmid

#211864

Purpose

Cas9 cutting at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

AAVS1

Use

CRISPR

Tags

Expression

Mutation

N/A

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3

Plasmid

#115521

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3 (Senp3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF_6xHIS_Csx29

Plasmid

#197613

Purpose

For protein purification of Csx29

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

6-HIS tagged Csx29

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_dCas7-11

Plasmid

#197605

Purpose

Expresses inactive Cas7-11 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

dCas7-11

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Gap43_Csx30_Cre

Plasmid

#197604

Purpose

Cre reporter for mammalian cells with Gap43 membrane anchor

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Gap43_Csx30_Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Cas7-11_T2A_Csx29

Plasmid

#197607

Purpose

Expresses Cas7-11 and Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Cas7-11_T2A_Csx29

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_HA_Csx30

Plasmid

#197610

Purpose

Expresses a HA-tagged version of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged Csx30

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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U6_crRNA_mCherry

Plasmid

#197609

Purpose

Expresses a Cas7-11 crRNA and mCherry

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

U6_crRNA_mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-JAK3(M511)_IRES_GFP

Plasmid

#206290

Purpose

Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (M511I)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Methionine 511 to Isoleucine

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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