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Plasmid#133869PurposeAtzA expression plasmidDepositorInsertAtzA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2
Plasmid#169245PurposeGeneric suicide vector for knockouts and integrationsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL7036
Plasmid#133871PurposeAtzC expression plasmidDepositorInsertAtzC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA1662
Plasmid#46878PurposeRetroviral vector, Hygromycin resistanceDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18-mini-Tn7T-Gm-LacZ30
Plasmid#65028PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRTagsExpressionBacterialMutationPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA
Plasmid#220185PurposesgRNA expression vector - pEM7 promoterDepositorInsertpEM7 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyC
Plasmid#220187PurposesgRNA expression vector - pBG42 promoterDepositorInsertpBG42 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD
Plasmid#220188PurposesgRNA expression vector - pBG28 promoterDepositorInsertpBG28 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBsaG4SPdX
Plasmid#108203PurposeG4S-PdXDepositorInsertPutidaredoxin
UseTagsExpressionBacterialMutationchanged Cysteine 73 to Serine and Cysteine 85 to …Promoterlac promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBsaP450camGG
Plasmid#85101PurposeP450cam (CYP101A1)DepositorInsertP450cam
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AmrR
Plasmid#51150DepositorInsertAmrR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23101-GFPuv
Plasmid#106388PurposepBBR1k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-con-GFPuv
Plasmid#106386PurposeConstitutive trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-GFPuv
Plasmid#106382PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR2k-GFPuv
Plasmid#106383PurposeAnhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertetAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasR
Plasmid#193629PurposeConstitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.DepositorInsertLasR
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP5372
Plasmid#128733PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA13-GG
Plasmid#208002Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP5371
Plasmid#128732PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR8k-GFPuv
Plasmid#106385PurposeL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23107-GFPuv
Plasmid#106389PurposepBBR1k-GFPuv with Anderson promoter J23107 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pP450cam
Plasmid#85085PurposeExpresses P450cam (CYP101A1) in E. coliDepositorInsertP450cam
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPdR
Plasmid#85083PurposeExpresses putidaredoxin reductase in E. coliDepositorInsertPutidaredoxin reductase
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-GFPuv
Plasmid#106384PurposeIPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEP101E
Plasmid#97387Purposebinary construct of cytosolic sfGFP1-10 for plantDepositorInsertsfGFP1-10OPT
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-phzSM
Plasmid#141105PurposeContains a phzS-phzM operon BioBrickDepositorInsertphzSM
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-phzAG
Plasmid#141106PurposeContains a seven gene phzABCDEFG operon BioBrickDepositorInsertphzABCDEFG
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63-GG
Plasmid#208015Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC-PLlambda-alkSm4-3
Plasmid#44383DepositorInsertAlkSm4-3
UseSynthetic BiologyTagsExpressionMutationQ410I, S470T, K546E, E775DPromoterPLlambdaAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBb(B5)8k-sfGFP
Plasmid#110140PurposeIPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 originDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA23-GG
Plasmid#208013Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-con-GFPuv
Plasmid#106387PurposeConstitutive LlacO-1 promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LasI
Plasmid#193628PurposeInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23109-GFPuv
Plasmid#106392PurposepBBR6k-GFPuv with Anderson promoter J23109 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
907L
Plasmid#160237PurposeFlightN-ttgR-VP16-Sv40-attBDepositorInsertsTtgR repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_pBen-BenR
Plasmid#128127PurposeExpressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.DepositorInsertBenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBenAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS23·Pm-GFP
Plasmid#122593PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNVL-pvdIJD
Plasmid#169872PurposeIntegration vector for deleting pvdIJD locus in P. putidaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-WbpB-His6
Plasmid#89711PurposeExpresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tagDepositorInsertWbpB (wbpB Pseudomonas aeruginosa)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only