Addgene

We narrowed to 17,148 results for: gateway

Showing: 11941 - 11960 of 17148 results

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-QK1

Plasmid

#16183

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Quaking (QKI Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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MAC_C_TYRO3

Plasmid

#187793

Purpose

MAC-tagged gene expression of human TYRO3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

TYRO3 (TYRO3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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CBS_pCSdest

Plasmid

#53909

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CBS (CBS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
p6993 MSCV-Neo C-HA 18E7

Plasmid

#163311

Purpose

Expresses HPV18 E7

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Use

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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YFP Gamma 3

Plasmid

#36103

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 3 (Gng3 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pEN_TT Tgfbr3-HA

Plasmid

#58497

Purpose

Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Entry vector

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pCPP5108

Plasmid

#128726

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5107

Plasmid

#128725

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 EBNA1BP2

Plasmid

#67072

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Luc-Pten-3'UTR

Plasmid

#64098

Purpose

Pten Luciferase reporter

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

Pten 3'UTR (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
pAD/CMV/V5-DEST-Rac1-2G

Plasmid

#66112

Purpose

Second generation Rac1 FRET biosensor for adenovirus production

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Adenoviral

Tags

His-Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV PrM

Plasmid

#145340

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS3

Plasmid

#145345

Purpose

Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS4A

Plasmid

#145346

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 STAT3

Plasmid

#67120

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT3 (STAT3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only