pEN_TT Tgfbr3-HA
(Plasmid
#58497)
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PurposeGateway entry vector for an inducible HA-tagged mouse Tgfbr3
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEN_TTmiRc2
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Backbone manufacturerAddgene #25752
- Backbone size w/o insert (bp) 4422
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTgfbr3_HA
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Alt nametransforming growth factor, beta receptor III
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SpeciesM. musculus (mouse)
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Insert Size (bp)2553
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GenBank IDNM_011578
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Entrez GeneTgfbr3 (a.k.a. 1110036H20Rik, AU015626, AW215636, TBRIII)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site Mfe1 (destroyed during cloning)
- 5′ sequencing primer pCEP-fwd
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN_TT Tgfbr3-HA was a gift from Kevin Janes (Addgene plasmid # 58497 ; http://n2t.net/addgene:58497 ; RRID:Addgene_58497) -
For your References section:
A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA. Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. 10.1038/ncb2930 PubMed 24658685