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Plasmid#119745PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
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mNeonGreen-JIP4
Plasmid#164621PurposeExpresses JIP4 in mammalian cells with a mNeonGreen tagDepositorInsertJIP4 (SPAG9 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-hDDI2-D252N_pET11a
Plasmid#169122Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM483
Plasmid#52987PurposeA secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensorDepositorInsertLactaherin (Mfge8 Mouse)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1(S94A)
Plasmid#59145PurposeYAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factorsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationS94APromoterCMVAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM656
Plasmid#53086PurposeA non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a controlDepositorInsertLactaherin (Mfge8 Mouse)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationH210PPromoterCAGAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK p2A mCherry
Plasmid#117693PurposeCAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs peptide
UseCre/LoxTagsHA / FLAGExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
TccCas13a Twin SUMO
Plasmid#199754PurposeExpresses codon optimized thermostabile TccCas13a in E.coliDepositorInsertTccCas13a
UseTagsSUMOExpressionBacterialMutationPromoterLacIAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _60-89
Plasmid#59144PurposeYAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factorsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationaa60-89 deletedPromoterCMVAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH1R-P2A-mCherry-N1
Plasmid#84330PurposeCo-expresses (untagged) Histamine-1 receptor and mCherryDepositorInsertH1R-P2A-mCherry (HRH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB31 (hANGPTL8-V5-His lenti)
Plasmid#109116PurposeV5 and His-tagged human ANGPTL8 in a lentiviral vectorDepositorInsertANGPTL8 (ANGPTL8 Human)
UseLentiviralTags6x His and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GATA2
Plasmid#131602Purposeexpression of human GATA2 in mammalian cellsDepositorInsertGATA2 (GATA2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞IRFP
Plasmid#154017PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertIRFP670
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β ctrl
Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.U6.shRNA-Sdc1-1376
Plasmid#195574PurposeExpresses shRNA to knockdown mouse Syndecan-1DepositorInsertshRNA, eGFP (Sdc1 Synthetic)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only