Addgene

Showing: 11881 - 11900 of 24311 results

Results per page:

Sort results by:

pT7II-2N4Rtau

Plasmid

#177653

Purpose

Recombinant protein synthesis of human Tau

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

Tau (2N4R) (MAPT Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GNSTM-3-Flag-10-Lamp2b-HA

Plasmid

#71293

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Use

Tags

FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3 Unc5B-FLAG

Plasmid

#72195

Purpose

Expresses full-length Unc5b with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b (Unc5b Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmirGLO-3'UTR mouse Tnf

Plasmid

#207127

Purpose

Luciferase vector containing 3'UTR for mouse Tnf

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Tumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

Plasmid

#225828

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Article

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

mVenus-FKBP-K7GEF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF-Frb-v1

Plasmid

#201923

Purpose

E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Inserts

FrbA
FrbB
FrbC
FrbD
FrbE

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 (modified), T7 (modified, moderate), T7 (modif…

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shSNAI1

Plasmid

#115467

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shSNAI1 (SNAI1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SKIV2L

Plasmid

#156014

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SKIV2L (SKIC2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

D3E

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrp2.3-AP-His

Plasmid

#71974

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.3 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eSpCas9_sgAAVS1

Plasmid

#199213

Purpose

All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

AAVS1-gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

human U6

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST TFR1

Plasmid

#169890

Purpose

Express TFR1 open reading frame. Lacks IRE regulatory elements

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

TFRC (TFRC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

TFRC cDNA, lacking 3'UTR regulatory elements

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ10878

Plasmid

#183964

Purpose

CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D156R, E292R, D235R, D350R

Promoter

CAG

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge