-
Plasmid#105765PurposeEncodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assayDepositorInsertFDFT1 3'UTR (FDFT1 Human)
UseLuciferaseTagsExpressionMutationNonePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-FKBP-TIAM1(DHPH)
Plasmid#176138PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST QKI
Plasmid#19891DepositorInsertquaking homolog, KH domain RNA binding (QKI Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo DEST (w18-1)
Plasmid#17390Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, NeoDepositorTypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PHOT1
Plasmid#209187PurposepENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tagsDepositorInsertAT3G45780.1 (PHOT1 Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-A1Up
Plasmid#16170DepositorInsertUbiquilin 4 (UBQLN4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS8
Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-STAT3-3xFlag
Plasmid#99260PurposeAdenoviral vector encoding Flag-tagged murine STAT3DepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAdenoviralTags3x-FlagExpressionMutationPromoterCMVAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-hM3Dq
Plasmid#114283PurposeTET-inducible expression of the hM3Dq proteinDepositorInserthM3Dq
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pCellFree_G03 BATF3
Plasmid#67050PurposeStandard for cell-free expression benchmarking.DepositorInsertBATF3 (BATF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9A(E195Q)-HA
Plasmid#209244PurposeMammalian expression of ATP9A mutant E195QDepositorInsertATP9A (ATP9A Human)
UseTagsHAExpressionMammalianMutationE195QPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 S
Plasmid#158371PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 S (S )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4C-GFP
Plasmid#67402PurposeMammalian expression of hArl4c with C-term GFP tagDepositorInsertARL7 (ARL4C Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP5
Plasmid#158356PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP5 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTet-SNAP-Centrin3
Plasmid#136806PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only