pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
(Plasmid
#159810)
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PurposeOptimized enzyme for genome editing in C. elegans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST R4-R3
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
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SpeciesC. elegans (nematode)
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MutationNot applicable
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See www.wormbuilder.org for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159810 ; http://n2t.net/addgene:159810 ; RRID:Addgene_159810) -
For your References section:
Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans. Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, Frokjaer-Jensen C. Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0. 10.1038/s41467-020-19898-0 PubMed 33298957