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pDONR221_SLC38A8

Plasmid

#132082

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A8 (SLC38A8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV10-mFoxP1-ES

Plasmid

#35169

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC24A2

Plasmid

#131966

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-myc3-Mdm2 D367E

Plasmid

#52057

Purpose

expression of non-cleavable Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 D367E (MDM2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

D367E

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC35A4

Plasmid

#132123

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A4 (SLC35A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35B1

Plasmid

#132099

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35B1 (SLC35B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSK+ chickBMPR-1B CA

Plasmid

#49528

Purpose

cloning vector for constitutively active chick BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Insert

BMPR-1B CA (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

constitutively active mutation (Q203D); also P2A,…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC35E1

Plasmid

#132098

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E1 (SLC35E1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Mutation

Promoter

LTR-driven expression

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pCDF1-MCS2-EF1-Puro-RGS7

Plasmid

#113728

Purpose

Express RGS7 in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

Plasmid

#160577

Purpose

35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31 PB (attP:TMtb:P35S:attB)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex15

Plasmid

#35166

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-lincRNA-RoR-sh1 (Linc-sh1)

Plasmid

#45764

Depositor

George Daley

Article

Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

Insert

lincRNA-RoR-shRNA1 (LINC-ROR Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP Mps1 DK

Plasmid

#63703

Purpose

Inducible expression of GFP-Mps1 DK fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 47-156, kinetochore binding domain

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35D3

Plasmid

#132110

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D3 (SLC35D3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-myc-CCR5-VC

Plasmid

#98966

Purpose

myc-tagged human CCR5 fused to C-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

Plasmid

#105644

Purpose

Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

CMV:ikk1FL(C178A)-tdTomato

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only