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Plasmid#177043PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoterDepositorInsertgeraniol synthase (CrGES) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0104
Plasmid#177038PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by 35S promoterDepositorInsertP19 suppressor of gene silencing from Tomato Bushy Stunt Virus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAP12008
Plasmid#177036PurposeLevel 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCATDepositorInsertCauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0061
Plasmid#177026PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertiridoid oxidase; CYP76A26
UseSynthetic BiologyTagsExpressionMutationamplified from cDNA, contains T359S relative to K…PromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0818
Plasmid#177020PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertgeranyl pyrophosphate synthase; geranyl diphosphate synthase
UseSynthetic BiologyTagsExpressionMutationtransit peptide removedPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R14A/K310A
Plasmid#162655PurposeExpresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-ORF68 C52A
Plasmid#162660PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cellsDepositorInsertORF68
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C52 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K435A
Plasmid#162651PurposeExpresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid K435 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K450A/R451A
Plasmid#162653PurposeExpresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-181-2xStrep
Plasmid#138444PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-181PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-191-2xStrep
Plasmid#138445PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-191PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-216-2xStrep
Plasmid#138446PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-216PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24-2xStrep
Plasmid#138435PurposeExpresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (G20V)
Plasmid#119308Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cellsDepositorInsertE6-AP I (G20V) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationG20VPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (C21Y)
Plasmid#119304Purposelentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cellsDepositorInsertE6-AP I (C21Y) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationC21YPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)
Plasmid#119313Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 55-376PromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)
Plasmid#119317Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cellsDepositorInsertE6-AP II (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-87PromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)
Plasmid#119310Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-64PromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only