PHAGE-P CMVt N-HA hE6-AP I (G20V)
(Plasmid
#119308)
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Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePHAGE
- Backbone size w/o insert (bp) 7818
- Total vector size (bp) 10374
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE6-AP I (G20V)
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Alt nameUBE3A
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SpeciesH. sapiens (human)
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Insert Size (bp)2556
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MutationG20V
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Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHAGE-P CMVt N-HA hE6-AP I (G20V) was a gift from Peter Howley (Addgene plasmid # 119308 ; http://n2t.net/addgene:119308 ; RRID:Addgene_119308) -
For your References section:
Angelman syndrome-associated point mutations in the Zn-binding N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding to the proteasome. Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM. J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 10.1074/jbc.RA118.004653. 10.1074/jbc.RA118.004653 PubMed 30257870