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Showing: 1161 - 1180 of 13188 results

  3. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  4. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172832-plasmid-map-sequence-id-340914

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  5. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  7. pX551-CMV-CjCas9

    Plasmid
    #107035
    Purpose
    AAV plasmid expressing CjCas9 under control of CMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107035-plasmid-map-sequence-id-208555

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  8. Cas9 gateway compatible

    Plasmid
    #128321
    Purpose
    Cas9 C-terminal gateway fusion
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128321-plasmid-map-sequence-id-255032

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  13. pAC84-pCR8-dCas9

    Plasmid
    #48218
    Purpose
    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A)
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48218-plasmid-map-sequence-id-69495

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  14. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP160
    Expression
    Mutation
    D10A;H840A nuclease-deficient
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48221-plasmid-map-sequence-id-69506

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  19. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet(Tetracycline inducible protein)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

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Showing: 1161 - 1180 of 13188 results