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Addgene
Showing: 1161 - 1180 of 13875 results

  2. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  3. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  6. pHOT-P2Y2R

    Plasmid
    #159108
    Purpose
    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson
    Depositor
    Fabio Mammano
    Article
    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc
    Insert
    P2Y2R
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    UBC
    Availability
    Academic Institutions and Nonprofits only
    159108-plasmid-map-sequence-id-318664

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  7. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid
    #182425
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    Use
    Tags
    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    182425-plasmid-map-sequence-id-360468

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  9. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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  14. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  15. TALEN Backbone

    Plasmid
    #31179
    Depositor
    Feng Zhang
    Insert
    TALE Nuclease Backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. pBAD33.1-DpnII

    Plasmid
    #127413
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Depositor
    Douglas Feldman
    Insert
    DpnII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Availability
    Academic Institutions and Nonprofits only
    127413-plasmid-map-sequence-id-247669

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  17. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165540-plasmid-map-sequence-id-339262

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  18. pmCer-RecQL4

    Plasmid
    #211400
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211400-plasmid-map-sequence-id-420508

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  19. pmCer-TopBP1

    Plasmid
    #211412
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211412-plasmid-map-sequence-id-420515

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  20. pmCer-RPA32

    Plasmid
    #211385
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211385-plasmid-map-sequence-id-420500

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Showing: 1161 - 1180 of 13875 results