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Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHluorin
Plasmid#70113PurposeExpression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidificationDepositorInsertlyso-pHluorin (Cd63 Aequorea victoria, Mouse)
UseTagsHA-Tag and pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…ExpressionMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorInsertPrime editor
UseCRISPRTagsnoExpressionMammalianMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN173
Plasmid#22012DepositorInsertbJunVN173 (Jun Aequorea Victoria, Rat)
UseTagsFLAGExpressionMammalianMutationc-Jun(257-338)-Venus(1-172)PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Myt1l
Plasmid#27152DepositorInsertMyt1l (Myt1l Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorInsertHuman PARP1 (PARP1 Human)
UseTagshistidine-tagExpressionBacterialMutationPolymorphism A762PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA
Plasmid#116771PurposeLentiviral expression of PIK3CADepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2CreERT2
Plasmid#13777PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cas9-gRNA-GFP
Plasmid#124770Purpose3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
c-Myc-5-HT2A
Plasmid#67944PurposeN-terminally c-myc tagged human 5-hydroxytryptamine receptor 2ADepositorInsertHTR2A (HTR2A Human)
UseTagsc-MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_IRES_mC_pGK_tBFP
Plasmid#79123PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch ReceptorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1
Plasmid#188567Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp1
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PtPuc3_diaCas9_sgRNA
Plasmid#109219PurposeEpisome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutumDepositorInsertsLHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterLHCF1 terminator, LHCF2 promoter, U6 3' regi…Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Dendra2
Plasmid#103967PurposeMammalian expression of fluorescent protein Dendra2 used for transfection efficiency controlDepositorInsertDendra2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1047
Plasmid#143749PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSNAI2 (SNAI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP11(x7)-Actin
Plasmid#181967PurposeHuman beta actin tagged with 7 copies of GFP11.DepositorInsertGFP11(x7)-Actin (ACTB )
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.1
Plasmid#102549Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV sgRNA Expression Plasmid
Plasmid#174540PurposeContains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.DepositorTypeEmpty backboneUseAAVTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-super TEV protease
Plasmid#171782PurposeExpresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.DepositorInsertMBP-sTEV
UseTagsArg6 tag and Internal His6 tagExpressionBacterialMutationNo internal TEV cleavage site between the MBP and…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon TVA-mCherry
Plasmid#131779PurposeINTRSECT 2.0DepositorInsertTVA-mCherry
UseAAV; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD-B
Plasmid#209861PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP B
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC101-AmpR EV
Plasmid#208184Purposeempty plasmid vector, non-fluorescentDepositorTypeEmpty backboneUseReporterTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
gGBEv6.3-SpCas9
Plasmid#202629Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationMPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorInsertXRCC4 (XRCC4 Human)
UseTagsGFP-2xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E545K
Plasmid#116485PurposeLentiviral expression of PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE545KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-mSTING-EGFP
Plasmid#214148PurposeRetroviral transduction of EGFP-tagged STINGDepositorInsertSTING (Sting1 Mouse)
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-Bmi1
Plasmid#31783DepositorInsertBmi1 (BMI1 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GR11
Plasmid#89105Purposemammalian expression of full-length human glucocorticoid receptor in pCMV5DepositorInserthuman glucocorticoid receptor (NR3C1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.UB
Plasmid#191556PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only