Showing: 11761 - 11780 of 13976 results
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Plasmid#133040PurposeExpresses TAT-Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
UseTagsHis8, TAT, and TEV protease cleavage siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas8b2
Plasmid#126482PurposeEncodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas8b2
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas7
Plasmid#126483PurposeEncodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas7
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas5
Plasmid#126484PurposeEncodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas5
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE
Plasmid#139086PurposeExpresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
Plasmid#140457PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pEMS1943
Plasmid#82565PurposeHigh copy number plasmid containing Ple266 (S100B MiniPromoter) insert.DepositorInsertPle266
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1958
Plasmid#82569PurposeHigh copy number plasmid containing PR2/3:Con MiniPromoter insert.DepositorInsertPRS2/3:Con
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationT230I (please see depositor comments below)PromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…ExpressionMutationPromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)
Plasmid#85179PurposeFor nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.DepositorInsertnuclear localization signal (nls) tagRFP
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
UseTags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) (pGEX2T(TEV))
Plasmid#80633PurposeResidues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222;PromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4C (216-233) (pGEX2T(TEV))
Plasmid#80635PurposeResidues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202DepositorInsertCHMP4C (CHMP4C Human)
UseTagsGSTExpressionBacterialMutationResidues 216-233PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11761 - 11780 of 13976 results