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Plasmid#99130PurposeAn AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancerDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9InsertNLS-mRuby2
UseAAVTagsExpressionMammalianMutationPromotermDLX5/6Available sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS15 (hANGPTL3-Strep)
Plasmid#109112PurposeExpresses StrepII-tagged human ANGPTL3 in mammalian cellsDepositorInsertANGPTL3 (ANGPTL3 Human)
UseTagsStrepIIExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2
Plasmid#136235PurposeExpresses human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
UseTagsStrep SumoExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1
Plasmid#214671PurposeBacterial expression of GST-tagged mouse GTSF1DepositorInsertGTSF1 (Gtsf1 Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorInsertSMC4-mAID-HaloTag (SMC4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertMTdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH2R-mCherry-N1
Plasmid#84326PurposeHistamine-2 receptor tagged with mCherryDepositorInsertH2R (HRH2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 WT
Plasmid#186467PurposeExpression of human MOSPD2 fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT
Plasmid#60814Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFP-NLS (SV40)ExpressionMammalianMutationPromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HiBiT-FZD7
Plasmid#195845PurposeExpresses human FZD7 with an N-terminal HiBiTDepositorInsertFZD7 (FZD7 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 tethered
Plasmid#49245Purposeexpresses two human WT p53s tethered together and also expresses EGFP in mammalian cellsDepositorInsertp53-p53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH3R-mCherry-N1
Plasmid#84327PurposeHistamine-3 receptor tagged with mCherryDepositorInsertH3R-mCherry (HRH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH4R-mCherry-N1
Plasmid#84328PurposeHistamine-4 receptor tagged with mCherryDepositorInsertH4R-mCherry (HRH4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InsertDIO-tTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sniper-Cas9
Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only