p11d-tRPA-32Asp8
(Plasmid #102617)

• Purpose: Expression of human RPA with aspartic acid substituted phosphorylation domain
• Depositing Lab: Marc Wold
• Publication: Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-91. Epub 2003 Jun 20.

Backbone

• Vector backbone: pET11d
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RPA1
• Species: H. sapiens (human)
• Entrez Gene: RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)

Gene/Insert 2

• Gene/Insert name: RPA3
• Entrez Gene: RPA3 (a.k.a. REPA3, RP-A p14)

Gene/Insert 3

• Gene/Insert name: RPA2
• Mutation: with aspartic acid substituted phosphorylation domain
• Entrez Gene: RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The RPA2 gene in this plasmid is a variant that also contains a T88I mutation.