Skip to main content
Addgene
Showing: 11641 - 11660 of 18816 results
  1. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid
    #131138
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and tdTomato
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. kanMX6-ins6

    Plasmid
    #195043
    Purpose
    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pLentiDP1.2

    Plasmid
    #171095
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2
    Depositor
    Insert
    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Emerald, Geminin, miniAID, mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pEGFP-NMHC-IIA-3xA

    Plasmid
    #101041
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.
    Depositor
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1915A, S1916A, S1943A
    Promoter
    CMV
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid
    #157813
    Purpose
    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
    Depositor
    Insert
    GFP-binding protein
    Use
    For drosophila cells
    Tags
    3xFlag
    Expression
    Insect
    Mutation
    Promoter
    metallothionein
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 11641 - 11660 of 18816 results