Showing: 11641 - 11660 of 13976 results
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Plasmid#196665PurposeExpression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsGST and TEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-distrepII
Plasmid#196667PurposeExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisEgf
Plasmid#173845PurposeEncoding dog Egf.DepositorInsertA cDNA of canis EGF.
UseTagsExpressionMammalianMutationR1127Q- please see dep. commentsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DrFLINC-AKAR-Kras
Plasmid#182334PurposeGenetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.DepositorInsertDrFLINC-AKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
Plasmid#182357PurposePositive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPeft-3AvailabilityAcademic Institutions and Nonprofits only -
pv7_CBX1_2xChromo_noEGFP
Plasmid#179395PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFPDepositorInsertCBX1_2xChromo_noEGFP
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pv7_MBD1_MBD_noEGFP
Plasmid#179406PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_164 SpCas9 R1333A/R1335A
Plasmid#179528PurposeFor bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 R1333A/R1335A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginines 1333 and 1335 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas9-Act3.0
Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ-SpRY-Act3.0
Plasmid#178957PurposeIt consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertSpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato
Plasmid#176704Purposeneural activity dependent release of sbGLuc from presynaptic vesiclesDepositorInsertsGaussia luciferase, slow burn
dTomato
UseAAVTagsP2A and hPOMC1-26ExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only
Showing: 11641 - 11660 of 13976 results