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CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171381

Purpose

Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13Y.1

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

Plasmid

#190112

Purpose

AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Inserts

MMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)

Use

AAV

Tags

bpNLS and bpNLS-P2A-eGFP

Expression

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

EFS and U6, H1

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Use

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph20_eGFP_CCR5TC

Plasmid

#187444

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-HsSyx-375-792

Plasmid

#179292

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Use

Tags

His6 + GST + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 375-792 from accession number NP_0010361…

Promoter

T7

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG1_M18

Plasmid

#82357

Purpose

Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR Q48

Plasmid

#86428

Purpose

Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

Androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Poly-glutamine expansion, G130D

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSSRB070_pAC8-hsCRBN

Plasmid

#218789

Purpose

Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Use

Tags

Flag-TEV-Spy

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG

Plasmid

#118625

Purpose

Overexpresses ERG protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (33-479) (ERG Human)

Use

Lentiviral

Tags

None and none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Con/Fon-GCaMP6M

Plasmid

#137119

Purpose

Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

Plasmid

#171382

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-REPAIRv2

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

Plasmid

#81021

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.G646 Wfs *12 truncation

Promoter

5’LTR

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pSLIK-IDH2-R172K-FLAG

Plasmid

#66807

Purpose

Tet-inducible expression of mutant IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLVNP2.0-SpCas9

Plasmid

#206880

Purpose

Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

SpCas9 (cas9 )

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR Q0

Plasmid

#86427

Purpose

Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the poly-glutamine expansion

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE_HRASV12_Hyg

Plasmid

#195143

Purpose

HRas G12V overexpression vector with hygroymycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

hRAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 V5 WT

Plasmid

#190568

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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