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Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…TagsExpressionMutationPromoterNoneAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
A3H HapI-Cas9n-UGI
Plasmid#119140PurposeBase editor made from APOBEC3H Haplotype IDepositorInsertAPOBEC3H Haplotype I-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub].1026C
Plasmid#112691Purposeexpress gRNA targeting bTub under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub]
UseCRISPRTagsExpressionMutationPromoterdU6-3Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-5
Plasmid#115626PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertNLS-nCas9-NLS-wtTadA-TadA7.10-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDK promoter dCas9
Plasmid#113657PurposedCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.DepositorInsertTDK gene promoter and dCas9
UseCRISPRTagsExpressionBacterialMutationPromoterTDK gene promoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-H1-sgRNA-hTZAP
Plasmid#87186PurposeguideRNA targeting exon1 of human TZAPDepositorInsertTZAP (ZBTB48 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterH1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1310
Plasmid#91034PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSPDepositorInsertTaCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0208
Plasmid#91004PurposeModule A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_3
Plasmid#73527PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_4
Plasmid#73528PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_2
Plasmid#73526PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R
Plasmid#228722PurposeExpresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2702-CMV-iNme2-ABE8e-nt
Plasmid#228723PurposeExpresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2 nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2700-CMV-Nme2-ABE8e-nt-E932R
Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R
Plasmid#228727PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2703-CMV-iNme2Smu-ABE8e-nt
Plasmid#228724PurposeExpresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2Smu nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R
Plasmid#228728PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R
Plasmid#228732PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2712-CMV-eNme2-C-ABE8e-i1-L10
Plasmid#228733PurposeExpresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid eNme-C nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R
Plasmid#228731PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only