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Plasmid#176272PurposeExpression of non-targeting sgRNA of CasMINIDepositorInsertCasMINI non-targeting sgRNA (sgLacZ) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pb-TRE3G-SpCas9-IRES-blast
Plasmid#195506PurposeInducible Piggybac vector expressing SpCas9 with Blasticidin selectionDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUbi-MAD7
Plasmid#170133PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-DelGFP
Plasmid#133302PurposeEGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBT-pcoCas9
Plasmid#52254Purposetransient expression of pcoCas9 gene in plant cellsDepositorInsertpcoCas9
UseCRISPR; Plant expressionTagsFLAG and NLSExpressionMutationPromoterhybrid constitutive promoter 35SPPDKAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TATV) (pY220)
Plasmid#89353PurposeExpresses humanized AsCpf1 variant that recognizes TATV PAMsDepositorInsertCpf1, RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorInsertCas9
UseCRISPR; Xenopus and zebrafish expressionTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMGS7 (AAVS1sgRNA)
Plasmid#126582PurposeA CRISPR/Cas9 construct to target the AAVS1 locus in humansDepositorInsertAAVS1 sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Cetn1/1
Plasmid#50718PurposepX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.DepositorInsertsCetn1 sgRNA1
humanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBh and hU6Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9
Plasmid#80425PurposeUsed for in vitro transcription of SpCas9 mRNA.DepositorInsertSpCas9
UseCRISPRTagsnoneExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PBE
Plasmid#98163PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseRiceTagsExpressionPlantMutationPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePPE-spG
Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpGCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 RNA Pol III promoter human snRNAAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW SpCas9
Plasmid#131506Purposelentiviral expression of SpCas9 under hUBC promotorDepositorInsertSpCas9
UseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:dCas9:nls:10P3
Plasmid#135982PurposeExpresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cellsDepositorInsertdCas9 fused to 10 copies of the P3 coiled-coil
UseCRISPRTagsHisExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only