Addgene

Showing: 11581 - 11600 of 156588 results

Results per page:

Sort results by:

pSW068

Plasmid

#140036

Purpose

KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

cLac143

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

Plasmid

#193294

Purpose

episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2 sgKEAP1-3

Plasmid

#186837

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Promoter

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pERM2-nhpSer

Plasmid

#201922

Purpose

Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Inserts

SepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep

Use

Tags

None

Expression

Bacterial

Mutation

E412P, E414F, T417K, P495M, I496W, F529S and H67R…

Promoter

GlnS (constitutive), OXB20 (constitutive), lpp (c…

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
RabV CVS-N2c(deltaG)-tdTomato

Plasmid

#73462

Purpose

Expresses tdTomato for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

tdTomato

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GFP10-ZIP

Plasmid

#182241

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP

Use

Tags

split-GFP10 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-cMYC

Plasmid

#216510

Purpose

Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

cMYC

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Duet-1-NONO(53-312)

Plasmid

#135442

Purpose

Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Non-POU domain-containing octamer-binding protein (NONO Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRPS2

Plasmid

#23622

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRPS2 (PRPS2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
HBM-Luc

Plasmid

#35155

Depositor

Linda Penn

Article

Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Insert

Myc promoter (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains 2.5 kb of the c-myc minimal promoter

Promoter

c-myc

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-MCU-EGFP

Plasmid

#181866

Purpose

Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV and Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

scramble

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-miRFP680-N1

Plasmid

#197229

Purpose

Expresses the fusion protein of H2B-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge