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Plasmid#140651PurposeBRD4 tagging CRISPRDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-MT-P2A
Plasmid#214896PurposeExpresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sitesDepositorInsertSPLICSFAST-LDh-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW240
Plasmid#170791PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolutionDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS_nanorod_105b
Plasmid#120347Purposephagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)DepositorInsertStaple sequences for a DNA origami nanorod
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS
Plasmid#199351PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFAExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFPL10a
Plasmid#98747PurposeCre-dependently expresses the fusion protein EGFPL10aDepositorHas ServiceAAV Retrograde and AAV5InsertEGFPL10a
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-myc-Skp2
Plasmid#19947DepositorInsertS-phase kinase-associated protein 2 (p45) (SKP2 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
E228Q Vps4A-HA
Plasmid#200087Purpose3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vectorDepositorInsertVPS4A (VPS4A Human)
UseLentiviralTags3xHAExpressionMammalianMutationE228QPromoterCMVAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 Spastin
Plasmid#134461PurposeM1 Spastin expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationMet to Ala change at amino acid 87PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3450
Plasmid#144926PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK1 (ELK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
N370S GBA pcDNA3.1
Plasmid#188583PurposeEctopic expression N370S GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationN370SPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCINeoFlag ALIX
Plasmid#89859PurposeExpresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili-Luc
Plasmid#48688Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
IL-2 promoter luciferase
Plasmid#12194DepositorInsertIL-2 promoter (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-C1
Plasmid#54842PurposeLocalization: C1 Cloning Vector, Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits