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Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)_quadruple_mutant
Plasmid#135441PurposeExpresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationL535A, L539A, L546A, M549APromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRA/PINCO
Plasmid#108938PurposeRetroviral expression of mRIN2 dRADepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationDeletion of RA domain (aa 751-836)PromoterLTRAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9/PINCO
Plasmid#108939PurposeRetroviral expression of mRIN2 dRHVPS9DepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterLTRAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D3UFL8
Plasmid#103095PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Double
Plasmid#100746PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with deletion of clathrin binding mo…PromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Y815A
Plasmid#100744PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with Y815A mutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)∆CBM
Plasmid#100745PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertbeta2 adaptin (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with deletion of clathrin binding mo…PromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-Rh159-HA
Plasmid#179344PurposeAll-in-on "tet-on" 3G lentiviral vector plasmid encoding rhesus cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA tag fused to its cytoplasmic tailDepositorInsertRh159
UseLentiviral; All in one tet-on lentiviral vector (…TagsHA tagExpressionMammalianMutationPromotertet responsive element 3GAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLM-AMA15.0 Cas9
Plasmid#138944PurposeFungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markersDepositorInsertp40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
UseCRISPR and Synthetic Biology; Ama1 autonomously r…TagsNLSExpressionMutationPromoterp40S (AN0465) Aspergillus nidulansAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HyPer7-MEM
Plasmid#136465PurposeMammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsFarnselation tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1
Plasmid#52326Purposeexpresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR117Q mutation (R95Q according to numbering in Fi…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPb-NLG in FSW lentiviral vector
Plasmid#73148PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.DepositorInsertsHRPb-NLG (Nlgn1 Mouse, Synthetic)
UseLentiviralTagsV5 tagExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV_CMV_CCR6-Nanoluc-V5
Plasmid#199672Purposeexpresses CCR6-NanoLuc-V5 in mammalian cells, AAV vectorDepositorInsertCCR6 (CCR6 Human)
UseAAVTagsNanoLuc and V5ExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorInsertRRBP1 (RRBP1 Human)
UseTagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …PromoterAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast
Plasmid#171951PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only