pLM-AMA15.0 Cas9
(Plasmid
#138944)
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PurposeFungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDSM-JAK-109
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Backbone manufacturerDSM
- Backbone size w/o insert (bp) 10170
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Modifications to backbonepGpda:DsRed-SKL removed
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Vector typeCRISPR, Synthetic Biology ; AMA1 autonomously replicating fungal plasmid
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Selectable markersFungal selection: Terbinafine (ergA) / Phleomycin/Bleomycin (ble)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
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SpeciesSynthetic; Fungal expression, Penicillium rubens
- Promoter p40S (AN0465) Aspergillus nidulans
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Tag
/ Fusion Protein
- NLS
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLM-AMA15.0 Cas9 was a gift from Arnold Driessen (Addgene plasmid # 138944 ; http://n2t.net/addgene:138944 ; RRID:Addgene_138944) -
For your References section:
CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. 10.1038/s41598-020-80864-3 PubMed 33441979