Addgene

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AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Article

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Use

Tags

6X Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pAN4023

Plasmid

#74701

Purpose

Output plasmid: PBM3R1-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PBM3R1-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Use

Tags

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pQacR-Q1

Plasmid

#74689

Purpose

QacR-Q1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ1-QacR-PQacR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-S3

Plasmid

#74693

Purpose

SrpR-S3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS3-SrpR-PSrpR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLEXY (pDN137)

Plasmid

#72656

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

mCherry-LEXY

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLitR-L1

Plasmid

#74683

Purpose

LitR-L1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSL1-LitR-PLitR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAmeR-F1

Plasmid

#74675

Purpose

AmeR-F1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSF1-AmeR-PAmeR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pmEOS2-VSVG

Plasmid

#98286

Purpose

mammalian expression of VSVG-mEOS2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

VSVG

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLmrA-N1

Plasmid

#74684

Purpose

LmrA-N1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSN1-LmrA-PLmrA-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-S2

Plasmid

#74692

Purpose

SrpR-S2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS2-SrpR-PSrpR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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APRT

Plasmid

#64028

Purpose

Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

Depositor

Vern Schramm

Article

Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

Insert

adenine phosphoribosyltransferase (APT1 Budding Yeast)

Use

Tags

Histidine x 6

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pSARM-luciferase

Plasmid

#67794

Purpose

Use together with VSV-G to package M-PMV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

firefly luciferase (gag )

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pPhlF-P3

Plasmid

#74687

Purpose

PhlF-P3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP3-PhlF-PPhlF-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBM3R1-B2

Plasmid

#74679

Purpose

BM3R1-B2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSB2-BM3R1-PBM3R1-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-P1

Plasmid

#74685

Purpose

PhlF-P1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP1-PhlF-PPhlF-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge