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Purpose(Empty Backbone) Multi-purpose rapid titer bacmam expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Cloning Grade DNA | 74099-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $105 |
Backbone
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Vector backboneModified pFastBacDual
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Backbone manufacturerThermo Fisher Scientific
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Modifications to backbonePolyhedrin promoter removed and replaced with CMV-containing cassette from pVLAD6 vector (Dukkipati et al., Protein Expr Purif., 2008). Multiple cloning site replaced with custom. Synthetic, codon-optimized EGFP inserted after p10 promoter. See Morales-Perez et al., Structure, 2016, for full detail on vector.
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Vector typeMammalian Expression ; Bacmam, Baculovirus
- Promoter CMV, p10
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Tag
/ Fusion Protein
- EGFP following p10 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byPartial sequence from pVLAD6 vector was used in assembling this new expression vector. pVLAD6 was a generous gift from Chris Garcia at Stanford University.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Information for Cloning Grade DNA (Catalog # 74099-DNA.cg) ( Back to top)
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $105 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEZT-BM was a gift from Ryan Hibbs (Addgene plasmid # 74099 ; http://n2t.net/addgene:74099 ; RRID:Addgene_74099) -
For your References section:
Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins. Morales-Perez CL, Noviello CM, Hibbs RE. Structure. 2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 10.1016/j.str.2016.03.004. 10.1016/j.str.2016.03.004 PubMed 27041595