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Showing: 1141 - 1160 of 15028 results

  1. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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  4. KBB430

    Plasmid
    #185091
    Purpose
    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    First nuclear localization signal mutated (amino …
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185091-plasmid-map-sequence-id-368167

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  5. KBB428

    Plasmid
    #185090
    Purpose
    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    First nuclear localization signal mutated (amino …
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185090-plasmid-map-sequence-id-367998

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  9. Banshee-ShCit-A

    Plasmid
    #85216
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-A
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85216-plasmid-map-sequence-id-171277

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  10. Banshee-ShCit-B

    Plasmid
    #85217
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-B
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85217-plasmid-map-sequence-id-171275

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  17. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Use
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  18. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  19. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  20. pOCC119

    Plasmid
    #118884
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mGFP, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118884-plasmid-map-sequence-id-232361

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Showing: 1141 - 1160 of 15028 results