Addgene

We narrowed to 2,484 results for: GUS

Showing: 1141 - 1160 of 2484 results

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pSpCas9(BB)-2A-Puro-SMCR8

Plasmid

#83440

Purpose

Expresses Cas9 and a gRNA targeting SMCR8

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human SMCR8 (SMCR8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FLCN

Plasmid

#49173

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-FLCN

Plasmid

#49174

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to tdTomato

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-C9orf72

Plasmid

#83439

Purpose

Expresses Cas9 and a gRNA targeting C9orf72

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human C9orf72 (C9orf72 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pQdC12a

Plasmid

#191356

Purpose

Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Inserts

PGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

PGus2A-TetO2/1 and miniPthl

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pQnl_tet

Plasmid

#191355

Purpose

Clostridium expression vector with TetR-repressed NanoLuc expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

PGus2A-TetO2/1

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHairyRed (double 35s removed)

Plasmid

#69710

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modified

Depositor

Brett Ferguson, Peter Gresshoff

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHIS2-KL EAT

Plasmid

#46042

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg1 EAT domain

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
punc-119cbr

Plasmid

#32568

Depositor

Al Fisher

Article

Ferguson et al Methods Mol Biol. 2013;940:87-102. doi:

Insert

unc-119

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG17-GFP

Plasmid

#44175

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg17 (ATG17 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

none

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
Atg17-Atg31-Atg29-pST39 (Auto 16)

Plasmid

#44174

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Inserts

Atg17
Atg31
Atg29

Use

Tags

HIS

Expression

Bacterial

Mutation

none

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
TFORF0747

Plasmid

#141682

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PIAS1 (PIAS1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3545

Plasmid

#145021

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PIAS1 (PIAS1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHairyRed

Plasmid

#69709

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots

Depositor

Brett Ferguson, Peter Gresshoff

Article

Lin et al Mol Plant. 2011 May;4(3):537-45. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pIV101

Plasmid

#196671

Purpose

Enables recombination mediated gene delivery into Agrobacterium strains for subsequent transformation of plants

Depositor

Simona Radutoiu

Article

Ferguson et al PLoS One. 2023 Nov 1;18(11):e0291680. do

Type

Empty backbone

Use

Integration vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Jun 8. ():.

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

Expression

Worm

Mutation

N/A

Promoter

Available since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1

Plasmid

#91942

Purpose

Bacterial expression of maize SAE1

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

HA-ZmSAE1

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Atg32-deltaPsR pXP721

Plasmid

#200735

Purpose

Plasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Use

Tags

GFP

Expression

Yeast

Mutation

Amino acids 200-341 are deleted.

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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