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  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful ...Figure 2). The GUS enzyme cleaves a substrate, which will then form a blue precipitate. GUS is usually the...check for GUS-like activity of your tissue. Run a control with Agrobacterium lacking the GUS gene since...endogenous GUS-like activity (Hu et al., 1990). We commonly use pSB161 - pL2_pSB90_2x35S::GUS::tMAS to ...example, we did our initial transformations with the GUS gene to see if the transformation was successful ...usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal reporters to measure...For example, we have seen strong expression of the GUS gene with the commonly used CaMV 35S promoter in ...

  3. Editor's Choice, August 2016

    Type
    Blog Post
    ...had quite the up-tick in views over the month of August - at over 56,000 views, we’ve grown 12% over last...surprising given how many scientists go on vacation in August (for another part of my job, I travel around the...non-vacationing) scientists. You can find all of our August posts in chronological order here: Site Directed...

  5. RUBY-Red Siliques

    Type
    Blog Post
    ...requiring the destruction of plants or tissue for GUS staining or are not suited to studying large samples...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...evidenced through Cre–loxP–mediated gene targeting. Gu, H., Zou, Y.R., and Rajewsky, K. 1993. PubMed PMID...

  12. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids Cloned by researcher Fluorescent protein, GUS, and LacZ Derivatives avaliable Philip Poole A family...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu, C., & Deverman, B. E. (2022). A high-efficiency ...effective in C57BL/6J mice, BALB/cJ mice, and cynomolgus macaques. MVD1A is effective in transducing brain...developed and deposited by the Sabeti lab for use in cynomolgus macaques. PAL1A, PAL1B, and PAL1C are effective...

  14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Competitive ELISA Similar...specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.       Direct ELISA In a direct...specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Indirect ELISA The indirect...specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Detecting the concentration... lab!   References and resources References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...40375 pBACgus/LRRK2 C-His LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40376 pBACgus/LRRK2...40385 pBACgus4x/Parkin PRKN polH Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...

  16. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk about,...gene mutation in human embryos." Nature. Epub 02 August 2017. doi:10.1038/nature23305. PubMed PMID: 28783728...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... al 2016 in a letter to Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED...Take Pooran's NgAgo Survey Survey results As of August 1, 2016, a total of 165 researchers responded to...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

  19. Synthetic Biology - Fungal

    Type
    Collection
    ...collection of synthetic biology plasmids for use in fungus....collection of synthetic biology plasmids for use in fungus. Fungal Plasmids Search the table by keyword or...
Showing: 1 - 20 of 57 results