Addgene

Showing: 1141 - 1160 of 18490 results

Results per page:

Sort results by:

pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Article

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Use

Tags

8xHIS and BPNLS

Expression

Bacterial

Mutation

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-TOMM20-N-10

Plasmid

#54282

Purpose

Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Insert

human androgen receptor (hAR) full-length (AR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Tsg101

Plasmid

#21505

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

Tsg101 (TSG101 Human)

Use

Tags

Cherry*

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-TGNP-N-10

Plasmid

#55145

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

TGNP (TGOLN2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Tags

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Use

Tags

MPB

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoEGFP

Plasmid

#46949

Purpose

GFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes

Depositor

John Schiller

Article

Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSU250

Plasmid

#173837

Purpose

Expresses 250 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-BRD7

Plasmid

#65379

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD7 (BRD7 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFDH

Plasmid

#131706

Purpose

Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

Depositor

Ron Milo

Article

Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

Insert

formate dehydrogenase

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge