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Plasmid#207753PurposeDonor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR-myr-AKT-HA
Plasmid#31790DepositorInsertAkt1 (Akt1 Mouse)
UseEntry vectorTagsHA and MyrExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiB
Plasmid#64900PurposeInducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.DepositorInsertNfiB (Nfib Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-AlkBH5(66-292)
Plasmid#125732PurposePlasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.DepositorInsertAlkBH5(66-292) (ALKBH5 Human)
UseTags6xHis and EINExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-mLin28A
Plasmid#26357DepositorInsertlin-28 homolog A (Lin28a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
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plenti-UBC- IKZF4-3xHA-pGK-PUR
Plasmid#107384PurposeLentiviral expression of IKZF4-3xHADepositorInsertIKZF4 (IKZF4 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221_SV2C
Plasmid#132297PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSV2C (SV2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A8
Plasmid#131989PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A8 (SLC22A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-hPPP3CA-FLAG
Plasmid#179134PurposeExpression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…PromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CAD
Plasmid#73566PurposeSoluble red fluorescent activator of Orai1 channelsDepositorInsertmCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
UseTags6x His and mCherryExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC35F2
Plasmid#132121PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC35F2 (SLC35F2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLL5.0 mCherry-hCortKD-Flcortmut3YD
Plasmid#187266PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF mCherry
Plasmid#187265PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgA1
Plasmid#187009PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/mCherry-DMDEx23
Plasmid#211367PurposeExpresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)DepositorInsertmCherry-DMDEx23
UseTagseGFPExpressionMammalianMutationmCherry interrupted by mdx dystrophin exon 23 bet…PromoterCMVAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only