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Plasmid#185289PurposeFor the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertiLID::eGFP::FTH1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
α-catenin ECFP-YPET FRET ΔF-actin binding site
Plasmid#198245PurposeAlpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.DepositorInsertα-catenin ECFP-YPET FRET sensor ΔF-actin binding site
UseTagsECFP and YPETExpressionMammalianMutationDeleted F-actin binding site on a-catenin (849-90…PromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28-C65A/C68A
Plasmid#92199PurposeMammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)DepositorInsertTif1b (TRIM28 Synthetic, Human)
UseTagsHAExpressionMammalianMutationChanged Cysteines 65 and 68 to Alanines.PromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-FBXL10
Plasmid#126542PurposeExpresses GFP-tagged FBXL10 in mammalian cellsDepositorInsertJHDM1B (KDM2B Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterLTRAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 1)-myc
Plasmid#62845PurposeMammalian Expression of myc-tagged Opa1 isoform1DepositorInsertmmOPA1isoform1-myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
UseTagsGLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK6-HA-TAK1
Plasmid#44160DepositorInsertTak1 (Map3k7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
UseTags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-eGFP
Plasmid#167585PurposeExpresses human CPSF6 fused to eGFPDepositorInsertCPSF6-eGFP (CPSF6 Human)
UseLentiviralTagseGFPExpressionMutationWTPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G228A
Plasmid#84926Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG228APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-iChloC_CA
Plasmid#98171Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseTagsmCherryExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/mCherry_pBirA-HA_FKBP8
Plasmid#154457Purposeexpresses mCherry_pBirA-HA_FKBP8/biochemical assayDepositorInsertmCherry_pBirA-HA_FKBP8 (FKBP8 Human)
UseTagsExpressionMammalianMutationPromoterCMV-FAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13-IDR-mCherry
Plasmid#145280PurposeBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion ProteinDepositorInsertHOXD13-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM24
Plasmid#65396PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM24 (TRIM24 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-AIM2
Plasmid#73958PurposeExpress c-Myc-tagged human AIM2 in mammalian cellsDepositorInsertabsent in melanoma 2 (AIM2) (AIM2 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-Cre
Plasmid#172433PurposeLentivirus, expresses Cre recombinaseDepositorInsertCre
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB kd K106R
Plasmid#108493Purposeexpression of EGFP-AuroraB kinase dead (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsEGFPExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-INK4A-zsGreen
Plasmid#110728PurposeMammalian Expression of p16INK4A IRES zsGreenDepositorInsertp16INK4A IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only