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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Gpat4-V5

Plasmid

#175145

Purpose

Lentiviral expression of mouse Gpat4-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Gpat4 (Gpat4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 3 kb prom. CD274

Plasmid

#107004

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Myc2- Hs TTP WT

Plasmid

#107008

Purpose

Expresses epitope-tagged wild-type human TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (ZFP36 Human)

Use

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-betaCatenin

Plasmid

#114281

Purpose

TET-inducible expression of a constitutively active form of the BetaCatenin protein

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

Catenin beta 1 (CTNNB1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

deletion of AA 1 to 45

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Dvl2

Plasmid

#123587

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

dishevelled segment polarity protein 2 (Dvl2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

Plasmid

#187154

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Pantelis Tsoulfas, Jae Lee

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTTCC

Plasmid

#164264

Purpose

Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

Depositor

Gudmundur Hreggvidsson

Article

Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

Inserts

Cas9
gRNA scaffold

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pcel and trc

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Use

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-PLOD2_PGK-GpNLuc

Plasmid

#136457

Purpose

Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)

Use

Luciferase and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-Rheb(S16H)

Plasmid

#194204

Purpose

Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

Depositor

Angelique Bordey

Article

Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

S16H

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM3D mCherry SV40

Plasmid

#92284

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM3D (CHRM3 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

GfaABC1D

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-BAFFR-CFP

Plasmid

#187002

Purpose

Expression of human BAFFR-CFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

BAFFR (TNFRSF13C Human)

Use

Lentiviral

Tags

CFP

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A6

Plasmid

#132196

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A6 (SLC6A6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-hCdt1-HA

Plasmid

#117500

Purpose

Expresses human Cdt1 fused to HA tag

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdc10-dependent transcript-1 (CDT1 Human, 1700 bp)

Use

Tags

HA

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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