Showing: 1121 - 1140 of 13875 results
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Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-dHeFSpCas9-VP64-6xHis
Plasmid#92119PurposeExpression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive HeFSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9 (without sgRNA)
Plasmid#92354PurposeExpression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
HeFSpCas9
Plasmid#92355PurposeExpression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E hTFNG
Plasmid#70130PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid, Asn413Asp, Asn…PromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorInsertNONO (NONO Human)
UseTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)-NLS
Plasmid#192443PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
Plasmid#167295PurposePlasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherryDepositorInsertBAK (BAK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374delNLS2
Plasmid#62532PurposeN-terminal aa1-269 and nuclear localization signal 2 of Drosha are deletedDepositorInserttruncated human Drosha mutant (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and nuclear localizatio…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailabilityAcademic Institutions and Nonprofits only -
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hFLT1 delta 754-763
Plasmid#86035Purposehuman VEGFR1 with a 10 amino acid deletion in EC close to TM domainDepositorInserthuman Flt1 delta754-763 aa (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationdelta 754-763PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
hFlt1 Y1333F
Plasmid#86037Purposehuman VEGFR1 with Y to F mutation at position 1333DepositorInserthuman Flt1 Y1333F (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationY1333F; HA tag is inserted downstream of signal …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-TfR
Plasmid#12130DepositorInserttransferrin receptor (TFRC Human)
UseTags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EXO1a
Plasmid#111629PurposeExpresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cellsDepositorInsertExonuclease 1a (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-SpCas9-HF1 (without sgRNA)
Plasmid#92102PurposeExpression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)DepositorInsert3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 1121 - 1140 of 13875 results