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Plasmid#118893Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC70
Plasmid#118878Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, cleavable with TEV protease; HIS6, cleavabl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hBnvy
Plasmid#18969DepositorInsertBrevican uncleavable (BCAN Human)
UseTagsExpressionMammalianMutationMutation at the ADAMTS cleavage site ESE/SRG: S…PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermQ3
Plasmid#51440PurposeExpresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Q3 C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ3
Plasmid#51441PurposeExpresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q3 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC204
Plasmid#118894Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and mKate2, HIS6,…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOE6089.4
Plasmid#135081PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-eGFP-Strep-tag II
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermCan
Plasmid#51438PurposeExpresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Can C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermCan
Plasmid#51439PurposeExpresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Can C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN1
Plasmid#51444PurposeExpresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC230
Plasmid#118871Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mCitrine, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC69
Plasmid#118874Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFP, cleava…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN1
Plasmid#51445PurposeExpresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N1 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_S235A
Plasmid#40639DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…ExpressionBacterialMutationS235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN2
Plasmid#51446PurposeExpresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150Q and K153QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN2
Plasmid#51447PurposeExpresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N2 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150Q and K153QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-G-G mm
Plasmid#221728PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.DepositorInsertBleoR-miR-430-G-G mm
UseFish expressionTagsExpressionMutationG-G mismatch of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-10-11 mm
Plasmid#221727PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.DepositorInsertBleoR-miR-430-10-11 mm
UseFish expressionTagsExpressionMutationMismatch at positions 10 and 11 of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-drAgo2
Plasmid#221711PurposeExpression of zebrafish Ago2 in zebrafish embryos.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseFish expressionTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-10xHis-ecRNHI*
Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
UseTagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only