We narrowed to 15,768 results for: LEA

1. pUCIDT-Dog1

   Plasmid

   #132765

   Purpose

   Encodes an iconic representation of a domestic dog

   Depositor

   Mark Bathe

   Article

   Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

   Insert

   Dog1
   

   Use

   Unspecified

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUCIDT-Dog2

   Plasmid

   #132766

   Purpose

   Encodes an iconic representation of a dometic dog

   Depositor

   Mark Bathe

   Article

   Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

   Insert

   Dog2
   

   Use

   Unspecified

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCIDT-Airplane1

   Plasmid

   #132767

   Purpose

   Encodes an iconic representation of an airplane

   Depositor

   Mark Bathe

   Article

   Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

   Insert

   Airplane1
   

   Use

   Unspecified

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUCIDT-Flower1

   Plasmid

   #132768

   Purpose

   Encodes an iconic representation of a flower

   Depositor

   Mark Bathe

   Article

   Banal et al Nat Mater. 2021 Sep;20(9):1272-1280. doi

   Insert

   Flower1
   

   Use

   Unspecified

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG vGlut1 mCherry

   Plasmid

   #105010

   Purpose

   Excitatory presynaptic marker

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   SLC17A7
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Tags

   A177C

   Promoter

   pBAD and pLac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin promoter

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVS2

    Plasmid

    #33144

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Inserts

    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    

    Expression

    Yeast

    Mutation

    GFP is mutated such that amino acids 64-73 (FSYGV…

    Promoter

    bidirectional GAL1-GAL10 promoter

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pPJ23116-MS2-rep

    Plasmid

    #184842

    Purpose

    Expression of the rep protein of phage MS2

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Replicase gene MS2 phage
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23116

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45564

    Purpose

    Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    d14 eGFP donor
    I-Sce I Nuclease
    BFP
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted n' 15 amino acids

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. P70a-deGFP-sc101-kan

    Plasmid

    #184841

    Purpose

    Expression of deGFP

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    promoter OR2-OR1

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KBB418

    Plasmid

    #185088

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS-nCas9n-A71

    Plasmid

    #125644

    Purpose

    micro-injection of poly-adenylated nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    nCas9n
    

    Use

    Rna in vitro transcription vector

    Promoter

    T3

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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