We narrowed to 15,678 results for: LEA

1. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pmCherry_gRNA

   Plasmid

   #80457

   Purpose

   gRNA expression vector with mCherry transfection control

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Inserts

   sp Cas9 gRNA
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin promoter

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. P70a-deGFP-sc101-kan

   Plasmid

   #184841

   Purpose

   Expression of deGFP

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   promoter OR2-OR1

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45564

   Purpose

   Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   d14 eGFP donor
   I-Sce I Nuclease
   BFP
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted n' 15 amino acids

   Promoter

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. KBB418

   Plasmid

   #185088

   Purpose

   Swi6L-GFP with nuclear export signal mutated to alanines

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Swi6 nuclear export signal L to A mutations

   Promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG vGlut1 GCaMP5G

   Plasmid

   #105008

   Purpose

   Presynaptic calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   SLC17A7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Use

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-(RTRG)6

    Plasmid

    #69849

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    6 RTRG repeats
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

    Plasmid

    #72240

    Purpose

    Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    GLT1 GAL1p 24xPP7 V3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrcHisA-NLS-BFP-mEosEM

    Plasmid

    #218934

    Purpose

    Express the nuclear import substrate NLS-BFP-mEosEM.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    BFP-mEosEM fusion protein
    

    Use

    Tags

    6xHis and SV40 nuclear localization sequence (NLS…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+-Zeo-miR-430

    Plasmid

    #221726

    Purpose

    Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE80l BCB

    Plasmid

    #196472

    Purpose

    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    BCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-N1-HsSmg6-mut-siRNAres_I

    Plasmid

    #146588

    Purpose

    Mammalian Expression of HsSmg6-mut-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

    Insert

    HsSmg6-mut-siRNAres (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. KBB416

    Plasmid

    #185087

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Promoter

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

    Plasmid

    #130678

    Purpose

    Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_F489del

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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