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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cabp5 (445bp crit)-tdTomato-IRES-AP

Plasmid

#18812

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Cabp5 (445bp crit)-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
Oct4-ires-EGFP(lox neo)

Plasmid

#21547

Depositor

Rudolf Jaenisch

Article

Lengner et al Cell Stem Cell. 2007 Oct 11;1(4):403-15.

Insert

EGFP, Oct4

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Gfi1-IRES-GFP

Plasmid

#91891

Purpose

Retroviral expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

MMLV gag

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-GFP-IRES-Pac

Plasmid

#48759

Purpose

CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

EGFP

Use

Ppy

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mRuby3-IRES-eGtACR1-ST

Plasmid

#108960

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-RA-HRas-IRES2-Lyn-iRFP682

Plasmid

#180584

Purpose

RA-Hras and Lyn-iRFP682

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

RA-Hras and Lyn-iRFP682

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D93A/D129A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

Plasmid

#198764

Purpose

Mammalian expression of hOCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 WT (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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CAG::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32480

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F:GlyR

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
CaM12(D20A/D56A)/pIRES2-eGFP

Plasmid

#111512

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-tdTomato-IRES-AP

Plasmid

#18809

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
hMYOG-3xHA-IRES-hrGFP (#324)

Plasmid

#78341

Purpose

Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

Depositor

Matthew Alexander, Louis Kunkel

Insert

MYOG (MYOG Human)

Use

Tags

3x HA tag and IRES-hrGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
MSCV Myc P57S IRES GFP

Plasmid

#18771

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc P57S (MYC Human)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

P57S

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
SV40 basal promoter-GFP-IRES-AP

Plasmid

#18807

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-GFP-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R536C-WLS-HA-IRES-GFP

Plasmid

#178065

Purpose

Expression of WLS-HA protein (R536C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Use

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

R536C

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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