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pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR
Plasmid#13507DepositorInsertFKHR (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA4
Plasmid#101043PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA4
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPIASx alpha
Plasmid#15209DepositorInsertPIASx alpha (PIAS2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMW#3
Plasmid#13350DepositorInsertLacZ reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
POLN_pDEST17
Plasmid#64885Purposebacterial expression of POLN for purificationDepositorInsertPOLN (POLN Human)
UseTagsFlag and HisExpressionBacterialMutationPromoterT7Available sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SpG‐ABE8e(V106W)
Plasmid#187069PurposeABE8e(V106W)-SpGDepositorInsertSpG‐ABE8e(V106W)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T4 DNA Ligase
Plasmid#206207PurposeInducible expression of T4 DNA ligaseDepositorInsertT4 DNA ligase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKO (Kan)
Plasmid#110086PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21
Plasmid#83421PurposeMouse Rab21 (wild type)DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-pBAD
Plasmid#54536PurposeLocalization: Protein Expression Vector , Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTags6xHis and mAppleExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mUbp43
Plasmid#12454DepositorInsertUBP43 (Usp18 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR2-EYFP
Plasmid#67877PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterUbCAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-PIK3R1
Plasmid#174192PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQdCas9-sgempty
Plasmid#159523PurposeExpression of dCas9 driven by lux cassette from Vibrio fischeriDepositorInsertquorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
BiP-mCherry
Plasmid#62233PurposeHamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.DepositorInserthamster BiP-mCherry-KDEL
UseTagsmCherryExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCiVSP(C363S)-SD64TF
Plasmid#80334PurposeExpresses Ciona VSP (without phosphatase activity) in Xenopus OocyteDepositorInsertCiona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
UseXenopus oocyte expressionTagsExpressionMutationChanged Cysteine 363 to SerinePromoterSP6Available sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3R1
Plasmid#174191PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1
Plasmid#204222PurposeExpression of Halo-Profilin-1 in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagC wt
Plasmid#19304DepositorInsertRas-related GTP binding C (RRAGC Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA wt
Plasmid#19298DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagD wt
Plasmid#19307DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-CFP
Plasmid#18827DepositorInsertTRPML1 (MCOLN1 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD268
Plasmid#132523PurposemNG^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mNGExpressionWormMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB wt
Plasmid#19301DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-ecfp-a
Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF-N-HiBiT-template
Plasmid#200882Purposetemplate for N-terminal HiBIT-taggingDepositorInsertHiBIT-linker
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-RFP
Plasmid#22907DepositorInserthuman Synapsin I promoter driving DsRedExpress (SYN1 Human)
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mTagBFP-T2A-TetOn3G
Plasmid#179888PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsmTagBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.dTomato
Plasmid#89395PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPIASx beta
Plasmid#15210DepositorInsertPIASx beta (PIAS2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CG
Plasmid#174189PurposeExpresses PIK3CG in mammalian cellsDepositorInsertPIK3CG (PIK3CG Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV.CAG.GLPLight1
Plasmid#187467PurposeExpresses GLPLight1 in mammalian cellsDepositorInsertGLPLight1
UseAAVTagsFlag tagExpressionMutationPromoterCAGAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SP-FRB (T2098L) -sfGFP-TM-V5
Plasmid#186227PurposemRNA synthesis of rapalog-induced cell adhesion moleculeDepositorInsertmRNA synthesis of rapalog-induced cell adhesion molecule
UseTagsV5ExpressionMammalianMutationFRB (T2098L)PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-mULK1M92A
Plasmid#31964DepositorInsertULK1 (Ulk1 Mouse)
UseTagsHAExpressionMammalianMutationM92APromoterCMV, SP6Available sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only
