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Addgene

pS2513·PHP
(Plasmid #122590)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122590 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSEVA2513
  • Backbone manufacturer
    SEVA
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6013
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    phIorine2
  • Species
    synthetic, based on superfolder GFP
  • Insert Size (bp)
    717
  • Promoter Pem7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer GCGGCAACCGAGCGTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS2513·PHP was a gift from Pablo Ivan Nikel (Addgene plasmid # 122590 ; http://n2t.net/addgene:122590 ; RRID:Addgene_122590)
  • For your References section:

    Non-invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator. Arce-Rodriguez A, Volke DC, Bense S, Haussler S, Nikel PI. Microb Biotechnol. 2019 Jun 4. doi: 10.1111/1751-7915.13439. 10.1111/1751-7915.13439 PubMed 31162835