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Plasmid#64788PurposeLuciferase expression driven by miR-34a promoter P4DepositorInsertmiR-34a promoter P4 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-WRAP53beta-siRNA-resistant
Plasmid#64680Purposeexpresses siRNA resistant WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsFLAGExpressionMammalianMutationsiRNA resistantPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHDm
Plasmid#64038PurposeMammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226, with aa 48–50 replaced b…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d3
Plasmid#59891Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756S
Plasmid#60730PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756S
Plasmid#60725PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-Q758C
Plasmid#60724PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-CHES1-ER
Plasmid#51846Purposefusion protein between CHES1 and the ligand binding domain of estrogen receptorDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsERExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1H164A
Plasmid#51844PurposeFlag-CHES1L mutant with ponctual mutation on histidine 164DepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC
Plasmid#51839PurposeFlag-CHES1L mutant lacking the C-terminal and NLS domainsDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationcontains CHES1 amino acids 1-205PromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dN
Plasmid#51843PurposeFlag-CHES1L mutant lacking the N-terminal domainDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationcontains CHES1 amino acids 110-490PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP45
Plasmid#41676DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; 45 C-terminal-…PromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP45
Plasmid#41675DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 45 C-terminal-…PromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP28
Plasmid#41673DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 28 C-terminal-…PromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRLCon1288-1666
Plasmid#31448DepositorInsertHNF4A 3'UTR fragment (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221_850-1207
Plasmid#31449DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon183-221mut2_850-1207
Plasmid#31450DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationmut2 in balancerPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRLCon1-449_850-1207
Plasmid#31451DepositorInsertHNF4A 3'UTR fragments (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -