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Plasmid#195540PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalExpressionMutationPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR77-DuET
Plasmid#213291PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR77 (C5AR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human FLAG-UAF1 S170Y
Plasmid#217619PurposeMammalian expression of FLAG tagged mutant (UAF1 S170Y) UAF1DepositorInsertUAF1 (WDR48 Human)
UseTagsExpressionMammalianMutationS170YPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple-NFAST(low)
Plasmid#214418PurposePeroxisome localization of low-affinity NFAST fused to PMP34-AppleDepositorInsertRspA-N (SLC25A17 )
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINOH11-A10
Plasmid#184278PurposeExpresses nanobody H11-A10 in E. coliDepositorInsertNanobody H11-A10
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11-B5
Plasmid#184279PurposeExpresses nanobody H11-B5 in E. coliDepositorInsertNanobody H11-B5
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_11-H6
Plasmid#184280PurposeExpresses nanobody H11-H6 in E. coliDepositorInsertNanobody H11-H6
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINH11-H4 Q98R_H100E
Plasmid#184281PurposeExpresses nanobody H11-H4 Q98R_H100Ein E. coliDepositorInsertNanobody H11-H4 Q98R_H100E
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(T59/T61A)(HA)
Plasmid#195025PurposeGateway vector containing HA-tagged Creb5 with T59/T61A mutationDepositorInsertHA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationT59/T61APromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(T59/T61D)(HA)
Plasmid#195026PurposeGateway vector containing HA-tagged Creb5 with T59/T61D mutationDepositorInsertHA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationT59/T61DPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Creb5(HA)
Plasmid#195024PurposeHA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vectorDepositorInsertHA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(T59/T61A)(HA)
Plasmid#195027PurposeHA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(T59/T61D)(HA)
Plasmid#195028PurposeHA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61DPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-W
Plasmid#200493PurposeLentiviral vector expressing gRNA targeting human RHOADepositorInsertRHOA(15) (RHOA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-W
Plasmid#208545PurposeLentiviral vector expressing gRNA targeting human TAL1DepositorInsertTAL1(11) (TAL1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W
Plasmid#200510PurposeLentiviral vector expressing gRNA targeting human SMARCA2DepositorInsertSMARCA2(47) (SMARCA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TAL1(12))-PGKpuro2ABFP-W
Plasmid#208546PurposeLentiviral vector expressing gRNA targeting human TAL1DepositorInsertTAL1(12) (TAL1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-W
Plasmid#200459PurposeLentiviral vector expressing gRNA targeting human AAVS1DepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MRPS26(15))-PGKpuro2ABFP-W
Plasmid#200499PurposeLentiviral vector expressing gRNA targeting human MRPS26DepositorInsertMRPS26(15) (MRPS26 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only