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Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Brainbow3.2-PGK-PuroTK
Plasmid#159928PurposeBrainbow transgenesisDepositorInsertsNFPnls
mOrange2
EGFP
mKate2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-MCS
Plasmid#80488Purposedonor vector for AAVS1 targeting (puromycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215326PurposeLentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertdNTP63 Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterdNTP63Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYC-C
Plasmid#207114PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ910
Plasmid#44481DepositorInsertMinimal Mos1, NeoR, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5T7-LYCipi-ggpps
Plasmid#122017PurposeExpresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.DepositorInsertGGPP synthase
UseTagsExpressionBacterialMutationcodon optimized, truncated first 98 amino acids, …PromoterT7Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Pa1
Plasmid#138475PurposeEnables high-efficiency recombineering in P. aeruginosaDepositorInsertsPapRecT
mutL E36K
UseTagsExpressionBacterialMutationE36KPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCherry-flex-dtA
Plasmid#58536PurposeExpresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cellsDepositorInsertdtA (DIP_RS18250 )
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215327PurposeLentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertMUC5AC Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterMUC5ACAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215328PurposeLentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertFOXJ1 Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterFOXJ1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-hTRIM21
Plasmid#104972PurposeLentiviral Transfer Plasmid with mCherry-tagged human TRIM21DepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only