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pLX317-EGFP

Plasmid

#115439

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

Plasmid

#218761

Purpose

Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

stGtACR1

Use

AAV

Tags

FusionRed

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHel2

Plasmid

#102960

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Article

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Plasmid shuttle vector system for genetic complem…

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pWS174

Plasmid

#90961

Purpose

Cas9 gap repair vector - NatR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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ER-SPLICSs-cFAST10

Plasmid

#210431

Purpose

Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

Depositor

Tito Calì

Insert

ER-SPLICSs-cFAST10

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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OMM-SPLICSs-nFAST

Plasmid

#210430

Purpose

Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

Depositor

Tito Calì

Insert

OMM-SPLICSs-nFAST

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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htrpC6-YFP

Plasmid

#21084

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

TrpC6 (TRPC6 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-Inframe-stop

Plasmid

#177620

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Expression

Mutation

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

Plasmid

#182358

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Pmex-5

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Otx2

Plasmid

#35001

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Homeobox protein OTX2 (Otx2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTRV2-GG

Plasmid

#105349

Purpose

virus-induced gene silencing

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

TRV-RNA2(5')

Use

In planta gene silencing; viral

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-Blue FP

Plasmid

#52115

Purpose

a bicistronic IRES-Blue FP containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-Blue FP

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG

Plasmid

#211549

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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