Showing: 11241 - 11260 of 13976 results
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Plasmid#98154PurposeExpresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695Q
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to glutaminePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695A
Plasmid#98155PurposeExpresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695A
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to alaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:Y696G
Plasmid#98156PurposeExpresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:Y696G
UseTagsHis6-TEVExpressionBacterialMutationChanged tyrosine 696 to glycinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
UseTagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pJG367
Plasmid#91185PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_H840A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJG382
Plasmid#91189PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+gNt_F2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAcrF1
Plasmid#89233PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF1
UseTags6xHis-TEV and FLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
UseTags6xHis-TEVExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
RF-211-UB-Lyn
Plasmid#79184PurposeExpression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertLyn (LYN Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-18PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorInsertsSpCas9
PmCDA1
UseTagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79621PurposeExpresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionMammalianMutationD10A and H840A for nuclease deficient Cas9PromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
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GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-204PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB
Plasmid#78348PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo
Plasmid#78352PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1
Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-Y705F-STAT3
Plasmid#71445PurposeRetroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationY705FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Sema3c(L)-AP-His
Plasmid#72015PurposeExpresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 11241 - 11260 of 13976 results