-
Plasmid#163343PurposeRetroviral vector to express cytosolic GFP and an HA-tagged puromycin selection markerDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159858PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
phFluc-WT
Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA S31N
Plasmid#19718DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationS31N mutant for dominant neg function. NOTE: lo…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFAB3689
Plasmid#47834PurposeBIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.DepositorInsertpromoter 3 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mCasp8
Plasmid#208782PurposeInducibly expresses mouse caspase8 fused to light-activatable Cry2DepositorInsertmCherry mCaspase8 Cry2 (Casp8 Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV-Beta(4)Integrin
Plasmid#16265DepositorInsertBeta 4 Integrin (ITGB4 Human)
UseTagsExpressionMammalianMutationlacked the 70- and 7-amino acid splice variants a…PromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAT9751-BEAR-mCherry-preedited
Plasmid#162993PurposeBEAR control plasmid with split mCherry and intact 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFAB3893
Plasmid#47844PurposeBIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.DepositorInsertpromoter 13 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1899)
Plasmid#170143PurposeExpresses residues 1-1899 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1900-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (1-1547)
Plasmid#170144PurposeExpresses residues 1-1547 of CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTagsFLAGExpressionInsectMutationC-terminal truncation of residues 1548-2997PromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7 (K999R)
Plasmid#170142PurposeExpresses CHD7 (K999R) in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationK999RPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159862PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_FL
Plasmid#59392PurposePlasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
Plasmid#159809PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only