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Plasmid#191973PurposeFRET-based indicator of ER-mitochondria proximity (FEMP)DepositorInsertAkapYFP-Tav2a-CFP-sac1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
UseTagsHis, MBP, StrepII, and bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD45
Plasmid#203753PurposeEncodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrCD45 (PTPRC Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
trPAGmEos3.2
Plasmid#203752PurposeEncodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrPAG (PAG1 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0770
Plasmid#177060PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertloganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD
Plasmid#168161PurposeInducible expression of PmcCAST TnsAB and TnsC proteins.DepositorInsertPmcCAST Tns proteins (TnsAB and TnsC)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS26: pTns(PmcCAST)_ΔTniQ
Plasmid#168159PurposeInducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.DepositorInsertPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS27: pTns(PmcCAST)_ΔTnsD
Plasmid#168160PurposeInducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS25: pTns(PmcCAST)
Plasmid#168158PurposeInducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA
Plasmid#201964PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationE162A,M164A,R167APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA
Plasmid#201965PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationE198A, R199A,E200A, D201APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA
Plasmid#201967PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR232A, K233A, R234A, K235A, R236APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA
Plasmid#201968PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationW255A,N262E,K266EPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 pET21a+
Plasmid#172321PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0116
Plasmid#177048PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0114
Plasmid#177046PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeraniol 8-oxidase (CrG8H) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0112
Plasmid#177044PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeraniol synthase (CrGES) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
D89_Ld_343450
Plasmid#135902PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserttransmembrane/endomembrane-like protein
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
UseTagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only